CD42b Rabbit Polyclonal Antibody
Catalog# ER1803-26
CD42b Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
unconjugated
Overview
Product Name
CD42b Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human CD42b aa 117-328 / 652.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 71 kDa
Positive Control
Jurkat, human spleen tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
WB
-
1:500
Target
Function
CD42a is a single-chain membrane glycoprotein that forms a noncovalent complex with CD42b. CD42b, also known as glycoprotein Ib a (GPIb a) is a membrane glycoprotein that is composed of a and b chains. The CD42b b chain is also designated CD42c, and is expressed on platelets and megakaryocytes. CD42a and CD42b are also present on platelets and mega-karyocytes, and the complex is a major component of the platelet surface. The complex acts as a receptor for von Willebrand's factor and as a von Willebrand's factor-dependent adhesion receptor.
Background References
暂无
Post-translational Modification
Glycocalicin is the product of a proteolytic cleavage/shedding, catalyzed by ADAM17, which releases most of the extracellular domain. Binding sites for vWF and thrombin are in this part of the protein.
Subcellular Location
Membrane. Secreted.
UNIPROT
Synonyms
Antigen CD42b alpha antibody
BDPLT1 antibody
BDPLT3 antibody
BSS antibody
CD 42b antibody
CD42b alpha antibody
CD42B antibody
CD42b antigen antibody
DBPLT3 antibody
GLYCOCALICIN antibody
ExpandImages
-
ICC staining CD42b in Jurkat cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD42b antibody (ER1803-26) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-26) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
