CD69 Mouse Monoclonal Antibody [E6-B6]
Catalog# EM1709-21
CD69 Mouse Monoclonal Antibody [E6-B6]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD69 Mouse Monoclonal Antibody [E6-B6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
30 kDa
Positive Control
Jurkat, L1210, THP-1 cell lysate, human tonsil tissue, Jurkat.
Conjugation
unconjugated
Clone Number
E6-B6
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:100-1:200
Target
Function
CD69 is expressed as a disulfide-linked homodimer called the activation inducer molecule (AIM), which is composed of two differentially glycosylated forms of a single protein. CD69 is among the earliest antigens to appear after activation of T cells, B cells and NK cells. CD69 is expressed constitutively on platelets, CD4+ or CD8+ thymocytes, and germinal center T cells, but is absent from resting lymphocytes.
Background References
1. Yokoyama WM et al. Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation. J Immunol 141:369-76 (1988).
2. Yokoyama WM et al. Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation. Immunol Rev 109:153-76 (1989).
Subcellular Location
Membrane.
UNIPROT
Synonyms
Activation inducer molecule (AIM/CD69) antibody
Activation inducer molecule antibody
AIM antibody
BL-AC/P26 antibody
BLAC/P26 antibody
C-type lectin domain family 2 member C antibody
CD69 antibody
CD69 antigen (p60
early T-cell activation antigen) antibody
CD69 antigen antibody
ExpandImages
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Western blot analysis of CD69 on different cell lysate using anti-CD69 antibody at 1/1,000 dilution.
Positive control:
Line1: Jurkat Line2: L1210 Line3: THP-1 -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD69 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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DEK terminates diapause by activation of quiescent cells in the crustacean Artemia
Journal: Biochemical Journal
DOI:
IF: 4.331
Application: WB
Reactivity: Artemia
Publish date: 2019 Jun
Alternative Products
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Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD69 Mouse Monoclonal Antibody [A8C4]
Application: WB
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD69 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated