CD69 Rabbit Polyclonal Antibody
Catalog# HA500073
CD69 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CD69 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CD69 aa 1-199.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
K562 cell lysate, mouse bone marrow tissue lysate, mouse spleen tissue lysate, HL-60 cell lysate, rat bone marrow tissue lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. It is an early activation marker that is expressed in hematopoietic stem cells, T cells, and many other cell types in the immune system. It is also implicated in T cell differentiation as well as lymphocyte retention in lymphoid organs.The activation of T lymphocytes and Natural Killer (NK) Cells, both in vivo and in vitro, induces expression of CD69. This molecule, which appears to be the earliest inducible cell surface glycoprotein acquired during lymphoid activation, is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, including natural killer (NK) cells, and platelets.
Background References
1. Natarajan K. et. al. Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells. Biochemistry 39:14779-14786(2000).
Subcellular Location
Membrane.
Synonyms
Activation inducer molecule (AIM/CD69) antibody
Activation inducer molecule antibody
AIM antibody
BL-AC/P26 antibody
BLAC/P26 antibody
C-type lectin domain family 2 member C antibody
CD69 antibody
CD69 antigen (p60, early T-cell activation antigen) antibody
CD69 antigen antibody
CD69 molecule antibody
ExpandImages
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Western blot analysis of CD69 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500073, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Mouse bone marrow tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: HL-60 cell lysate -
Western blot analysis of CD69 on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500073, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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VSIG2 as a novel immunosuppressive ligand interacts with Nectin-2 to regulate T cell responses
Journal: Journal Of Neuroinflammation
DOI: 10.1186/s12974-025-03645-7
IF: 10.1
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec
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VSIG2 as a novel immunosuppressive ligand interacts with Nectin-2 to regulate T cell responses
Journal: Research Square
DOI:
IF:
Application: WB
Reactivity: Mouse
Publish date: 2024 Dec
Alternative Products
CD69 Mouse Monoclonal Antibody [A8C4]
Application: WB
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD69 Mouse Monoclonal Antibody [E6-B6]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated