CD69 Mouse Monoclonal Antibody [A8C4]
Catalog# HA601013
CD69 Mouse Monoclonal Antibody [A8C4]
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WB
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Human
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unconjugated
Overview
Product Name
CD69 Mouse Monoclonal Antibody [A8C4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD69 aa 62-199/199.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Daudi cell lysate treated with PMA for 24 hours.
Conjugation
unconjugated
Clone Number
A8C4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. It is an early activation marker that is expressed in hematopoietic stem cells, T cells, and many other cell types in the immune system. It is also implicated in T cell differentiation as well as lymphocyte retention in lymphoid organs. The activation of T lymphocytes and Natural Killer (NK) cells, both in vivo and in vitro, induces expression of CD69. This molecule, which appears to be the earliest inducible cell surface glycoprotein acquired during lymphoid activation, is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, including natural killer (NK) cells, and platelets. CD69 expression has been associated with both regulatory T cell (Treg), memory T cell and Bcl6 loCD69 hiLZ GC B plasmablast precursors. In addition to Treg differentiation, CD69 is a common marker of precursor and mature resident memory T cells (TRMs) that are localized in peripheral tissues. TGF-β is also responsible for the development of TRMs, thus promoting TRM differentiation in a manner similar to Treg differentiation. Most lymphocytes express sphingosine-1-phosphate receptors (S1P1-5), which are G protein-coupled receptors located in the cell membrane that bind to the ligand sphingosine-1-phosphate (S1P). S1P is a sphingolipid metabolite that is abundant in the bloodstream and, upon binding to S1P1, promotes lymphocyte egress from lymphoid organs so they can travel to affected tissues.
Background References
1. You Z. et. al. The Clinical Significance of Hepatic CD69(+) CD103(+) CD8(+) Resident-Memory T Cells in Autoimmune Hepatitis. Hepatology. 2021 Aug
2. Gorabi AM. et. al. The pivotal role of CD69 in autoimmunity. J Autoimmun. 2020 Jul
Subcellular Location
Membrane.
UNIPROT
Synonyms
Activation inducer molecule (AIM/CD69) antibody
Activation inducer molecule antibody
AIM antibody
BL-AC/P26 antibody
BLAC/P26 antibody
C-type lectin domain family 2 member C antibody
CD69 antibody
CD69 antigen (p60, early T-cell activation antigen) antibody
CD69 antigen antibody
CD69 molecule antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of CD69 on different lysates with Mouse anti-CD69 antibody (HA601013) at 1/500 dilution.
Lane 1: Daudi cell lysate
Lane 2: Daudi cell lysate treated with PMA for 24 hours
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD69 Mouse Monoclonal Antibody [E6-B6]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
