CLIC2 Rabbit Polyclonal Antibody
Catalog# ER2001-41
CLIC2 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CLIC2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CLIC2 aa 80-247.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, FC
Target Molecular Weight
28 kDa
Positive Control
K562 cell lysate, mouse lung tissue lysate, K562.
Conjugation
unconjugated
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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FC
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1:50-1:100
Target
Function
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.Expressed in adult and fetal brain, heart, skeletal muscle, liver, lung, and spleen. Detected in adult stomach and testis. Expressed in fetal thymus and kidney.
Background References
1. Board P.G. et. al. CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels. Int. J. Biochem. Cell Biol. 36:1599-1612(2004).
Subcellular Location
Cytoplasm, Membrane.
Synonyms
Chloride intracellular channel 2 antibody
Chloride intracellular channel protein 2 antibody
CLIC 2b antibody
CLIC2 antibody
CLIC2_HUMAN antibody
CLIC2b antibody
MRXS32 antibody
OTTHUMP00000196471 antibody
XAP 121 antibody
XAP121 antibody
Images
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Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Western blot analysis of CLIC2 on different lysates with Rabbit anti-CLIC2 antibody (ER2001-41) at 1/2,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-41, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 28 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CLIC2 Mouse Monoclonal Antibody [A5G10]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
