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CLIC2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-41

CLIC2 Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

CLIC2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human CLIC2 aa 80-247.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Molecular Weight

28 kDa

Positive Control

K562 cell lysate, mouse lung tissue lysate, K562.

Conjugation

unconjugated

RRID

AB_3069518

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.Expressed in adult and fetal brain, heart, skeletal muscle, liver, lung, and spleen. Detected in adult stomach and testis. Expressed in fetal thymus and kidney.

Background References

1. Board P.G. et. al. CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels. Int. J. Biochem. Cell Biol. 36:1599-1612(2004).

Subcellular Location

Cytoplasm, Membrane.

UNIPROT

SwissProt: O15247 Human

SwissProt: Q5M883 Rat

Synonyms

Chloride intracellular channel 2 antibody

Chloride intracellular channel protein 2 antibody

CLIC 2b antibody

CLIC2 antibody

CLIC2_HUMAN antibody

CLIC2b antibody

MRXS32 antibody

OTTHUMP00000196471 antibody

XAP 121 antibody

XAP121 antibody

Images

  • Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-41" style="font-weight: bold;text-decoration: underline;">ER2001-41</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: K562 cell lysate<br />Lane 2: Mouse lung tissue lysate

    Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: K562 cell lysate
    Lane 2: Mouse lung tissue lysate

  • Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-41" style="font-weight: bold;text-decoration: underline;">ER2001-41</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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