iFluor™ 488 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]
Catalog# HA720159F
iFluor™ 488 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]
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IF-Cell
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FC
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Human
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human E-Cadherin aa 591-640 / 882.
Species Reactivity
Human
Validated Applications
IF-Cell, FC
Molecular Weight
Predicted band size: 97 kDa
Positive Control
MCF-7, A431.
Conjugation
iFluor™ 488
Clone Number
SY0287
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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FC
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1:50-1:1,000
Target
Function
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function.
Background References
1. Su B et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016).
2. Schmidt TP et al. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA. Sci Rep 6:23264 (2016).
Subcellular Location
Endosome, Cell membrane, trans-Golgi network, adherens junction.
UNIPROT
Synonyms
Arc 1 antibody
CADH1_HUMAN antibody
Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin1 antibody
CAM 120/80 antibody
CD 324 antibody
CD324 antibody
CD324 antigen antibody
cdh1 antibody
ExpandImages
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Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Flow cytometric analysis of A431 cells labeling E-Cadherin.
Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of MCF-7 cells labeling E-Cadherin.
Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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