Mammalian Insulin enzyme-linked immunoassay kit
Catalog# EH0038
Mammalian Insulin enzyme-linked immunoassay kit
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ELISA
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Mammalian
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31.25~2000 pg/ml
Overview
Product Name
Mammalian Insulin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
20.89 pg/ml (50 μl) ；28.28 pg/ml (20 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0076 | 0.0079 | |
| 31.25 | 0.0100 | 0.0101 | 0.0101 | 0.0022 |
| 62.50 | 0.0122 | 0.0127 | 0.0125 | 0.0046 |
| 125.00 | 0.0245 | 0.0242 | 0.0244 | 0.0165 |
| 250.00 | 0.0767 | 0.0754 | 0.0761 | 0.0682 |
| 500.00 | 0.3493 | 0.3107 | 0.3300 | 0.3222 |
| 1000.00 | 1.2640 | 1.1620 | 1.2130 | 1.2052 |
| 2000.00 | 3.2150 | 3.1260 | 3.1705 | 3.1627 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 581.0 | 2316.1 | 7446.9 | 579.8 | 2277.0 | 7736.2 |
| Standard Deviation | 12.8 | 34.2 | 243.7 | 14.4 | 34.5 | 230.5 |
| Coefficient of Variation (%) | 2.2 | 1.5 | 3.3 | 2.5 | 1.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammalian Insulin into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 110%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | n.d-289.05 | 90 | 41.29 |
| Rat Serum | 30 | 13.76-924.50 | 100 | 106.12 |
| Human Serum | 30 | 132.03-2758.63 | 100 | 1027.08 |
Serum/Plasma – Thirty samples from apparently healthy mice/healthy volunteers/healthy rats were evaluated for the presence of Mammalian Insulin in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃