Mammalian GFAP enzyme-linked immunoassay kit
Catalog# EH0178
Mammalian GFAP enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mammal
Standard curve range
-
68.59~50000 pg/ml
Overview
Product Name
Mammalian GFAP enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.52 pg/ml (50 μl)；17.06 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0335 | 0.0361 | 0.0348 | |
| 68.59 | 0.0570 | 0.0603 | 0.0587 | 0.0239 |
| 205.76 | 0.0843 | 0.0939 | 0.0891 | 0.0543 |
| 617.28 | 0.1807 | 0.1806 | 0.1807 | 0.1459 |
| 1851.85 | 0.4176 | 0.4205 | 0.4191 | 0.3843 |
| 5555.56 | 1.1570 | 1.1750 | 1.1660 | 1.1312 |
| 16666.67 | 2.8440 | 2.9960 | 2.9200 | 2.8852 |
| 50000.00 | 4.3945 | 4.5339 | 4.4642 | 4.4294 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 724.3 | 3800.8 | 14128.0 | 794.5 | 4527.5 | 16907.8 |
| Standard Deviation | 2.2 | 17.4 | 76.9 | 6.7 | 25.3 | 78.4 |
| Coefficient of Variation (%) | 4.7 | 6.5 | 5.2 | 3.8 | 5.4 | 5.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的哺乳动物 GFAP，未加人 GFAP 的血清作为本底，计算回收率。回收率的范围从 96 % 至 104 %，平均回收率为 101 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.29 | 75 | 5.29 |
应用本试剂盒，检测健康哺乳动物的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mammal
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
wu:fb34h11
ALXDRD
cb345
etID36982.3
FLJ42474
FLJ45472
GFAP
GFAP_HUMAN
gfapl
Glial fibrillary acidic protein
ExpandFunction
GFAP（神经胶质纤维酸性蛋白）是一种III型中间丝蛋白。它是星形胶质细胞中间丝的主要成分。GFAP 缺陷是亚历山大病的一个原因。亚历山大病是一种罕见的中枢神经系统疾病。它是一种进行性白质脑病，其标志是罗森塔尔纤维的广泛积累，罗森塔尔纤维是星形胶质细胞中的细胞质包涵体。在氨基酸序列水平上，人GFAP与大鼠和小鼠GFAP分别具有91%和90%的同一性。