Mammalian CD62P/P-Selectin enzyme-linked immunoassay kit
Catalog# EH0289
Mammalian CD62P/P-Selectin enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Mammalian CD62P/P-Selectin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.19 pg/ml (50 μl)；70.99 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0444 | 0.0441 | 0.0443 | |
| 156.25 | 0.1587 | 0.1492 | 0.1540 | 0.1097 |
| 312.50 | 0.2757 | 0.2684 | 0.2721 | 0.2278 |
| 625.00 | 0.4935 | 0.4962 | 0.4949 | 0.4506 |
| 1250.00 | 0.8932 | 0.8620 | 0.8776 | 0.8334 |
| 2500.00 | 1.4950 | 1.5650 | 1.5300 | 1.4858 |
| 5000.00 | 2.3990 | 2.4770 | 2.4380 | 2.3938 |
| 10000.00 | 3.3780 | 3.4450 | 3.4115 | 3.3673 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 199.2 | 1022.8 | 3359.7 | 161.6 | 695.0 | 2182.5 |
| Standard Deviation | 10.0 | 44.6 | 381.0 | 7.6 | 29.3 | 104.0 |
| Coefficient of Variation (%) | 5.0 | 4.4 | 11.3 | 4.7 | 4.2 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mammalian CD62P/P-Selectin into health mammalian serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 7.85-29.14 | 100 | 18.17 |
| Mouse Serum | 30 | 355.11-903.67 | 100 | 572.82 |
| Rat Serum | 30 | 26.18-72.12 | 100 | 46.71 |
| Monkey Serum | 1 | 4.32 | 100 | 4.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, thirty samples from apparently healthy mice, thirty samples from apparently healthy rats and one sample from healthy monkeys were evaluated for the presence of mammalian CD62P/P-Selectin in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃