Mammalian VEGF-C enzyme-linked immunoassay kit
Catalog# EH0113
Mammalian VEGF-C enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mammal
Standard curve range
-
13.72~10000 pg/ml
Overview
Product Name
Mammalian VEGF-C enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.82 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0193 | 0.0193 | 0.0193 | |
| 13.72 | 0.0278 | 0.0271 | 0.0275 | 0.0082 |
| 41.15 | 0.0444 | 0.0434 | 0.0439 | 0.0246 |
| 123.46 | 0.0984 | 0.0931 | 0.0958 | 0.0765 |
| 370.37 | 0.2400 | 0.2467 | 0.2434 | 0.2241 |
| 1111.11 | 0.6659 | 0.6791 | 0.6725 | 0.6532 |
| 3333.33 | 1.7940 | 1.8210 | 1.8075 | 1.7882 |
| 10000.00 | 3.8780 | 3.7850 | 3.8315 | 3.8122 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 240.1 | 1112.3 | 3324.8 | 233.3 | 1079.7 | 3258.5 |
| Standard Deviation | 12.3 | 50.9 | 159.1 | 7.2 | 37.5 | 71.2 |
| Coefficient of Variation (%) | 5.1 | 4.6 | 4.8 | 3.1 | 3.5 | 2.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康哺乳动物血清加入 3 个不同浓度水平的哺乳动物VEGF-C，未加哺乳动物 VEGF-C 的血清作为本底，计算回收率。回收率的范围从 80 % 至 110 %，平均回收率为 91 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 474.36-2997.14 | 100 | 1487.71 |
应用本试剂盒，检测健康哺乳动物的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mammal
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Flt 4L
Flt4 ligand
FLT4 ligand DHM
Flt4-L
Flt4L
Vascular endothelial growth factor C
Vascular endothelial growth factor related protein
Vascular endothelial growth factor-related protein
VEGF C
VEGF-C
ExpandFunction
血管内皮生长因子 C （VEGF-C） 是 VEGF R3/Flt-4 的同型二聚体配体，由 N 端和 C 端前肽合成。仅包含 VEGF 同源结构域的完全加工 VEGF-C 还可以结合和激活 VEGF R2/KDR/Flk-1。VEGF-C 与 VEGF R3 的相互作用对淋巴管生成至关重要。配体和受体通常在淋巴管发芽部位、胚胎和各种病理条件下共表达。VEGF-C 在肿瘤细胞中过表达会诱导肿瘤淋巴增生、新血管生成和血管发芽，导致淋巴血流增强并转移至区域淋巴结。