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Osteoprotegerin Mouse Monoclonal Antibody [9C1]

Usd: 350

Specification

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Catalog# EM1701-98

Osteoprotegerin Mouse Monoclonal Antibody [9C1]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Osteoprotegerin Mouse Monoclonal Antibody [9C1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human Osteoprotegerin aa 1-401 / 401.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 46 kDa

Positive Control

MG-63 cell lysate, Human liver tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, mouse kidney tissue, 293.

Conjugation

unconjugated

Clone Number

9C1

RRID

AB_3068821

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:50

Target

Function

Bone morphogenesis and remodeling involve the formation of bone from osteoblasts and the resorption of bone by osteoclasts. The cytokine osteoprotegerin (OPG), also designated osteoclastogenesis inhibitory factor (OCIF), is known to inhibit osteoclast formation. A secreted glycoprotein, OPG is a member of the TNF receptor family that increases bone density and volume. OPG is thought to inhibit osteoclastogenesis by disrupting the cell-to-cell signaling between osteoblastic stromal cells and osteoclast progenitors. OPG is known to bind to TRAIL, a death domain-containing protein, and to inhibit TRAIL apoptosis in Jurkat cells. OPG also binds to osteoclast differentiation factor (ODF), also known as TRANCE/RANKL, a membrane-bound protein belonging to the TNF ligand family. Both TNFα and TNFβ upregulate OPG expression, while the bone resorbing agent prostaglandin E2 downregulates OPG.

Background References

1. Tsuda E et al. Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis. Biochem Biophys Res Commun 234:137-142 (1997).

2. Luan X et al. Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin. J Immunol 189:245-252 (2012).

Tissue Specificity

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

Post-translational Modification

N-glycosylated. Contains sialic acid residues.; The N-terminus is blocked.

Subcellular Location

Secreted.

UNIPROT

SwissProt: O00300 Human

SwissProt: O08712 Mouse

SwissProt: O08727 Rat

Synonyms

MGC29565 antibody

OCIF antibody

OPG antibody

Osteoclastogenesis inhibitory factor antibody

Osteoprotegerin antibody

PDB5 antibody

TNF receptor superfamily member 11b antibody

TNFRSF 11B antibody

TNFRSF11B antibody

TR 1 antibody

Expand

Images

  • Western blot analysis of Osteoprotegerin on different lysates with Mouse anti-Osteoprotegerin antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/2,000 dilution.<br /><br />Lane 1: MG-63 cell lysate<br />Lane 2: Human liver tissue lysate<br />Lane 3: Mouse kidney tissue lysate<br />Lane 4: Rat heart tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Osteoprotegerin on different lysates with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/2,000 dilution.

    Lane 1: MG-63 cell lysate
    Lane 2: Human liver tissue lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Rat heart tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-98) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Osteoprotegerin antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (<a href="/products/EM1701-98" style="font-weight: bold;text-decoration: underline;">EM1701-98</a>) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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