Cathepsin D Mouse Monoclonal Antibody [13F2]
Catalog# EM1901-15
Cathepsin D Mouse Monoclonal Antibody [13F2]
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WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
Cathepsin D Mouse Monoclonal Antibody [13F2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Cathepsin D aa 1-412 / 412.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 45 kDa
Positive Control
MCF7 cell lysate, U-937 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, Human liver tissue lysate, human breast cancer tissue, human liver cancer tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
13F2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:1,000
Target
Function
Cathepsin D is a protein that in humans is encoded by the CTSD gene. The main function of cathepsin D is to degrade proteins and activate precursors of bioactive proteins in pre-lysosomal compartments. This proteinase, which is a member of the peptidase A1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of the CTSD gene is initiated from several sites, including one that is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. Homozygous deletion of the CTSD gene leads to early lethality in the postnatal phase. Over-expression of cathepsin D stimulates tumorigenicity and metastasis as well as initiation of tumor apoptosis. This protease has been regarded an independent marker of poor prognosis in breast cancer being correlated with the incidence of clinical metastasis. Knock-out of CTSD gene would cause intestinal necrosis and hemorrhage and increase apoptosis in thymus, indicating that cathepsin D is required in certain epithelial cells for tissue remodeling and renewal.
Background References
1. Marques ARA. et. al. Enzyme replacement therapy with recombinant pro-CTSD (cathepsin D) corrects defective proteolysis and autophagy in neuronal ceroid lipofuscinosis. Autophagy. 2019 Jul 16:1-15.
2. Xi J. et. al. Absence of association of the Ala58Val (rs17571) CTSD gene variant with Parkinson\'s disease or amyotrophic lateral sclerosis in a Han Chinese population. Neurosci Lett. 2018 Jan 1;662:181-184.
Sequence Similarity
Belongs to the peptidase A1 family.
Tissue Specificity
Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post-translational Modification
N- and O-glycosylated.; Undergoes proteolytic cleavage and activation by ADAM30.; As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Subcellular Location
Lysosome, extracellular space, melanosome.
UNIPROT
Synonyms
CatD antibody
CATD_HUMAN antibody
Cathepsin D antibody
Cathepsin D heavy chain antibody
CD antibody
Ceroid lipofuscinosis neuronal 10 antibody
CLN10 antibody
CPSD antibody
ctsd antibody
Epididymis secretory sperm binding protein Li 130P antibody
ExpandImages
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Western blot analysis of Cathepsin D on different lysates with Mouse anti-Cathepsin D antibody (EM1901-15) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: U-937 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45/28 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cathepsin D antibody (EM1901-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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