CD40 Mouse Monoclonal Antibody [A1E5]

Western blot analysis of CD40 on different lysates with Mouse anti-CD40 antibody (EM1901-41) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Daudi cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 37 seconds; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1901-41, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 31 kDa
Observed band size: 42 kDa

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD40 antibody (EM1901-41) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD40 antibody (EM1901-41) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-CD40 antibody (EM1901-41) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (EM1901-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.