USP28 Mouse Monoclonal Antibody [A1F8]
Catalog# EM1901-47
USP28 Mouse Monoclonal Antibody [A1F8]
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WB
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Human
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unconjugated
Overview
Product Name
USP28 Mouse Monoclonal Antibody [A1F8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human USP28 aa 509-860 / 1,077.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 122 kDa
Positive Control
K562 cell lysates, A549 cell lysates.
Conjugation
unconjugated
Clone Number
A1F8
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
Background References
1. Serra R.W. et. al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 3:E02313-E02313(2014).
2. Bassermann F. et. al. The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint. Cell 134:256-267(2008).
Sequence Similarity
Belongs to the peptidase C19 family. USP28 subfamily.
Post-translational Modification
Degraded upon nickel ion level or hypoxia exposure.; Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.
Subcellular Location
Nucleoplasm.
UNIPROT
Synonyms
Deubiquitinating enzyme 28 antibody
KIAA1515 antibody
Ubiquitin carboxyl terminal hydrolase 28 antibody
Ubiquitin carboxyl-terminal hydrolase 28 antibody
ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
Ubiquitin specific peptidase 28 antibody
Ubiquitin specific processing protease 28 antibody
Ubiquitin specific protease 28 antibody
Ubiquitin thioesterase 28 antibody
Ubiquitin thiolesterase 28 antibody
ExpandImages
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Western blot analysis of USP28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
USP28 Rabbit Polyclonal Antibody
Application: WB,FC
Reactivity: Human
Conjugate: unconjugated
