YKL-40 / CHI3L1 Mouse Monoclonal Antibody [A3G10]
Catalog# EM1902-31
YKL-40 / CHI3L1 Mouse Monoclonal Antibody [A3G10]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
YKL-40 / CHI3L1 Mouse Monoclonal Antibody [A3G10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within extracellular domain of Human CHI3L1.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 43 kDa
Positive Control
THP-1 cell lysates, human tonsil tissue.
Conjugation
unconjugated
Clone Number
A3G10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Background References
1. Qiu QC. et. al. CHI3L1 promotes tumor progression by activating TGF-β signaling pathway in hepatocellular carcinoma. Sci Rep. 2018 Oct
2. Abd El-Fattah AA. et. al. Single Nucleotide Polymorphism in SMAD7 and CHI3L1 and Colorectal Cancer Risk. Mediators Inflamm. 2018 Oct
Sequence Similarity
Belongs to the glycosyl hydrolase 18 family.
Tissue Specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Post-translational Modification
Glycosylated.
Subcellular Location
Extracellular space, Endoplasmic reticulum, Cytoplasm.
UNIPROT
Synonyms
39 kDa synovial protein antibody
ASRT7 antibody
Cartilage glycoprotein 39 antibody
CGP-39 antibody
CGP39 antibody
CH3L1_HUMAN antibody
CHI3L1 antibody
chitinase 3 like 1 (cartilage glycoprotein 39) antibody
chitinase 3 like 1 antibody
Chitinase 3 like protein 1 precursor antibody
ExpandImages
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Western blot analysis of YKL-40 / CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"