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CD34 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER0802

CD34 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Zebrafish

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD34 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human CD34 aa 336-385 / 385.

Species Reactivity

Human, Mouse, Rat (Predicted: Zebrafish)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 41 kDa

Positive Control

TF-1 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, TF-1, human tonsil tissue, human liver carcinoma tissue.

Conjugation

unconjugated

RRID

AB_3069015

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:250

  • IHC-P

  • 1:2,000-1:3,000

  • FC

  • 1:1,000

Target

Function

CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.

Background References

1. "Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells." Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. J. Immunol. 148:267-271(1991)

2. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2004)

3. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2008)

Sequence Similarity

Belongs to the CD34 family.

Tissue Specificity

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational Modification

Highly glycosylated.; Phosphorylated on serine residues by PKC.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P28906 Human

Synonyms

CD34 antibody

CD34 antigen antibody

CD34 molecule antibody

CD34_HUMAN antibody

Cluster designation 34 antibody

Hematopoietic progenitor cell antigen CD34 antibody

HPCA1 antibody

Mucosialin antibody

OTTHUMP00000034733 antibody

OTTHUMP00000034734 antibody

Images

  • Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (<a href="/products/ER0802" style="font-weight: bold;text-decoration: underline;">ER0802</a>) at 1/2,000 dilution.<br /><br />Lane 1: TF-1 cell lysate (15 µg/Lane)<br />Lane 2: Human brain tissue lysate (20 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 100~120 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER0802" style="font-weight: bold;text-decoration: underline;">ER0802</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (ER0802) at 1/2,000 dilution.

    Lane 1: TF-1 cell lysate (15 µg/Lane)
    Lane 2: Human brain tissue lysate (20 µg/Lane)
    Lane 3: Mouse brain tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 100~120 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of TF-1 cells labeling CD34 with Rabbit anti-CD34 antibody (<a href="/products/ER0802" style="font-weight: bold;text-decoration: underline;">ER0802</a>) at 1/250dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD34 antibody (<a href="/products/ER0802" style="font-weight: bold;text-decoration: underline;">ER0802</a>) at 1/250dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of TF-1 cells labeling CD34 with Rabbit anti-CD34 antibody (ER0802) at 1/250dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD34 antibody (ER0802) at 1/250dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. Counter stained with hematoxylin.

  • Flow cytometric analysis of TF-1 cells labeling CD34.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ER0802" style="font-weight: bold;text-decoration: underline;">ER0802</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of TF-1 cells labeling CD34.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ER0802, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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