PTP1B Rabbit Polyclonal Antibody
Catalog# ER1802-83
PTP1B Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
PTP1B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human PTP1B aa 1-50 / 435.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 50 kDa
Positive Control
Hela cell lysate, A431 cell lysate, HUVEC, LOVO, human tonsil tissue, human colon cancer tissue, human kidney tissue, human placenta tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:200-1:500
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IHC-P
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1:50-1:1,000
Target
Function
The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
Background References
暂无
Sequence Similarity
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Tissue Specificity
Expressed in keratinocytes (at protein level).
Post-translational Modification
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Subcellular Location
Endoplasmic reticulum, Membrane.
UNIPROT
Synonyms
PTP1B antibody
Non receptor tyrosine phosphatase 1 antibody
Protein phosphotyrosylphosphatase 1B antibody
Protein tyrosine phosphatase 1B antibody
Protein tyrosine phosphatase non receptor type 1 antibody
Protein tyrosine phosphatase placental antibody
Protein-tyrosine phosphatase 1B antibody
PTN1_HUMAN antibody
PTP 1B antibody
PTP-1B antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of PTP1B on different lysates with Rabbit anti-PTP1B antibody (ER1802-83) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PTP1B KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-83) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PTP1B on different lysates with Rabbit anti-PTP1B antibody (ER1802-83) at 1/500 dilution.
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-83) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining PTP1B in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining PTP1B in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PTP1B antibody (ER1802-83) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-83) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PTP1B antibody (ER1802-83) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-83) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PTP1B Recombinant Rabbit Monoclonal Antibody [JJ0935]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Zebrafish
Conjugate: unconjugated
Products with the same target and pathway
PTP1B Mouse Monoclonal Antibody [D0-C7]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated