Antithrombin III / ATIII / SERPINC1 Rabbit Polyclonal Antibody
Catalog# ER1803-06
Antithrombin III / ATIII / SERPINC1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Antithrombin III / ATIII / SERPINC1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human SERPINC1 aa 150-370 / 464.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
53 kDa
Positive Control
Human plasma lysates, H22, HepG2, rat kidney tissue, human liver tissue, human small intestine tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:50,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
The protein encoded by this gene, antithrombin III, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. Numerous mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency which constitutes a strong risk factor for thrombosis. A reduction in the serum level of this protein is associated with severe cases of Coronavirus Disease 19 (COVID-19).
Background References
1. Sungurlu S et al. Role of Antithrombin III and Tissue Factor Pathway in the Pathogenesis of Sepsis. Crit Care Clin. 2020 Apr
2. Farrell DH et al. Antithrombin III Levels and Outcomes Among Patients With Trauma. JAMA Netw Open. 2024 Aug
Sequence Similarity
Belongs to the serpin family.
Tissue Specificity
Found in plasma.
Post-translational Modification
Phosphorylated by FAM20C in the extracellular medium.
Subcellular Location
Secreted.
Synonyms
ANT3_HUMAN antibody
Antithrombin antibody
Antithrombin III antibody
Antithrombin-III antibody
AntithrombinIII antibody
AT 3 antibody
AT III antibody
AT3 antibody
AT3D antibody
ATIII antibody
ExpandImages
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Western blot analysis of Antithrombin III / ATIII / SERPINC1 on human plasma lysate with Rabbit anti-Antithrombin III / ATIII / SERPINC1 antibody (ER1803-06) at 1/50,000 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1803-06, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 53 kDa
Observed band size: 53 kDa -
ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Antithrombin III / ATIII / SERPINC1 Mouse Monoclonal Antibody [15F2]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Antithrombin III / ATIII Recombinant Rabbit Monoclonal Antibody [PSH12-13]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Antithrombin III / ATIII / SERPINC1 Mouse Monoclonal Antibody [15F1]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
Products with the same target and pathway
Antithrombin III / ATIII / SERPINC1 Mouse Monoclonal Antibody [15F2]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated