CD40 Rabbit Polyclonal Antibody (ER1803-54)
Catalog# ER1803-54
Rabbit Polyclonal to CD40
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
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Mouse
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-54) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CD40 Rabbit Polyclonal Antibody (ER1803-54)
Immunogen
Recombinant protein within human cd40 aa 1-213 / 277.
Host
Rabbit
Positive Control
Daudi cell lysates, SiHa, rat spleen tissue, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50 -1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD40
SYNONYMS
AI326936 antibody;B cell associated molecule CD40 antibody;B cell surface antigen CD40 antibody;B cell-associated molecule antibody;B-cell surface antigen CD40 antibody;Bp50 antibody;CD 40 antibody;CD40 antibody;CD40 antigen (TNF receptor superfamily member 5) antibody;CD40 antigen antibody;CD40 molecule antibody;CD40 molecule, TNF receptor superfamily member 5 antibody;CD40 protein antibody;CD40 type II isoform antibody;CD40L receptor antibody;CDw40 antibody;GP39 antibody;HIGM1 antibody;IGM antibody;IMD3 antibody;MGC9013 antibody;Nerve growth factor receptor related B lymphocyte activation molecule antibody;OTTHUMP00000031699 antibody;OTTHUMP00000031700 antibody;p50 antibody;T-BAM antibody;TBAM antibody;TNF receptor superfamily member 5 antibody;TNFRSF5 antibody;TNR5_HUMAN antibody;TRAP antibody;Tumor necrosis factor receptor superfamily , member 5 antibody;Tumor necrosis factor receptor superfamily member 5 antibody;Tumor necrosis factor receptor superfamily member 5 precursor antibody;Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
TISSUE SPECIFICITY
B-cells and in primary carcinomas.
SUBCELLULAR LOCATION
Plasma membrane. Secreted.
FUNCTION
Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. A receptor ligand pair central to the trans-mission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif.
CITATIONS
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Xia, H., Wang, Y., Dai, J., Zhang, X., Zhou, J., Zeng, Z., & Jia, Y.
Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells
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WB
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Mouse
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Citation
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