Skip to content

NKG2D Rabbit Polyclonal Antibody

Usd:

Bulk Order Quote | Customization Request

Catalog# ER1913-86

NKG2D Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NKG2D Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human NKG2D 121-219/219

Species Reactivity

Human, Mouse

Validated Applications

WB, FC

Molecular Weight

25 KDa

Positive Control

Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • FC

  • 2μg/Test

Target

Function

Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.

Background References

暂无

Subcellular Location

Membrane; Single-pass type II membrane protein.

UNIPROT

SwissProt: P26718 Human

Synonyms

KLRK1

natural killer cell group 2D

NKG2-D isoform b

Klrk1

Nkrp2

NKG2-D type II integral membrane protein

NKG2-D-activating NK receptor

NK cell receptor D

Killer cell lectin-like receptor subfamily K

NK lectin-like receptor

Expand

Images

  • Sample: Lung (Mouse) Lysate at 40 ug<br />Primary: Anti- NKG2D (<a href="/products/ER1913-86" style="font-weight: bold;text-decoration: underline;">ER1913-86</a>) at 1/300 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 25 kD<br />Observed band size: 26 kD

    Sample: Lung (Mouse) Lysate at 40 ug
    Primary: Anti- NKG2D (ER1913-86) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 25 kD
    Observed band size: 26 kD

  • Blank control (blue line): Mouse thymus cells (blue). <br />Primary Antibody (green line): Rabbit Anti-NKG2D antibody (<a href="/products/ER1913-86" style="font-weight: bold;text-decoration: underline;">ER1913-86</a>) <br />Dilution: 1μg /10^6 cells; <br />Isotype Control Antibody (orange line): Rabbit IgG . <br />Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<br />Dilution: 1μg /test. <br />Protocol<br />The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Blank control (blue line): Mouse thymus cells (blue).
    Primary Antibody (green line): Rabbit Anti-NKG2D antibody (ER1913-86)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

NKG2D Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human

Conjugate: unconjugated

Products with the same target and pathway

metafield image

NKG2D Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human

Conjugate: unconjugated

metafield image

Biotin Conjugated Human NKG2D Recombinant Rabbit Monoclonal Antibody [PSH17-05] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

Human NKG2D Recombinant Rabbit Monoclonal Antibody [PSH17-04] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated

metafield image

Human NKG2D Recombinant Rabbit Monoclonal Antibody [PSH17-05] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated