Nucleoporin p62 Rabbit Polyclonal Antibody
Catalog# ER2001-57
Nucleoporin p62 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
Nucleoporin p62 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Nucleoporin p62 aa 470-522.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 53 kDa
Positive Control
293T cell lysate, HCT 116 cell lysate, Jurkat cell lysate, A549 cell lysate, HeLa cell lysate, PC-12 cell lysate, human breast carcinoma tissue, human rectum tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:100-1:400
Target
Function
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.
Background References
1. Carmo-Fonseca M. et. al. Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur. J. Cell Biol. 55:17-30(1991).
Subcellular Location
Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus.
UNIPROT
Synonyms
62 kDa nucleoporin antibody
DKFZp547L134 antibody
FLJ20822 antibody
FLJ43869 antibody
IBSN antibody
MGC841 antibody
Nuclear pore glycoprotein p62 antibody
nucleoporin 62kDa antibody
Nucleoporin Nup62 antibody
nucleoporin p62 antibody
ExpandImages
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Western blot analysis of Nucleoporin p62 on different lysates with Rabbit anti-Nucleoporin p62 antibody (ER2001-57) at 1/5,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: HCT 116 (Human colon cancer cell) cell lysate
Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 4: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 180 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-57, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 53.3 kDa
Observed band size: 62 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Nucleoporin p62 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-57, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-Nucleoporin p62 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-57, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Nucleoporin p62 Recombinant Rabbit Monoclonal Antibody [JE55-09]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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