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GFP Recombinant Rabbit Monoclonal Antibody [SR48-02]

Usd: 385

Specification

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Catalog# ET1602-7

GFP Recombinant Rabbit Monoclonal Antibody [SR48-02]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GFP Recombinant Rabbit Monoclonal Antibody [SR48-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.

Species Reactivity

Species independent

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 27 kDa

Positive Control

Recombinant GFP protein, GFP Tag fusion protein lysate.

Conjugation

unconjugated

Clone Number

SR48-02

RRID

AB_3069664

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

  • IHC-P

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IP

  • 2-5 µg/ml

Target

Function

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

Background References

1. "Primary structure of the Aequorea victoria green-fluorescent protein."Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.Gene 111:229-233(1992).

2. "A molecular thermometer based on fluorescent protein blinking."Wong F.H., Banks D.S., Abu-Arish A., Fradin C.J. Am. Chem. Soc. 129:10302-10303(2007).

UNIPROT

SwissProt: P42212 Aequorea victoria

Synonyms

GFP antibody

Green fluorescent protein antibody

yfp antibody

Images

  • Western blot analysis of GFP on recombinant GFP protein using anti-GFP antibody at 1/5,000 dilution.

    Western blot analysis of GFP on recombinant GFP protein using anti-GFP antibody at 1/5,000 dilution.

  • GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with <a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/M1004-8" style="font-weight: bold;text-decoration: underline;">M1004-8</a> at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: GFP Tag fusion protein lysate (input).<br />Lane 2: Rabbit IgG instead of <a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a> in GFP Tag fusion protein lysate.<br />Lane 3: <a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a> IP in GFP Tag fusion protein lysate.<br />Lane 4: <a href="/products/ET1604-25" style="font-weight: bold;text-decoration: underline;">ET1604-25</a> IP in GFP Tag fusion protein lysate.<br />Lane 5: <a href="/products/ET1604-26" style="font-weight: bold;text-decoration: underline;">ET1604-26</a> IP in GFP Tag fusion protein lysate.<br />Lane 6: <a href="/products/ET1607-31" style="font-weight: bold;text-decoration: underline;">ET1607-31</a> IP in GFP Tag fusion protein lysate.<br />Lane 7: <a href="/products/R1312-2" style="font-weight: bold;text-decoration: underline;">R1312-2</a> IP in GFP Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1602-7 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.

    Lane 1: GFP Tag fusion protein lysate (input).
    Lane 2: Rabbit IgG instead of ET1602-7 in GFP Tag fusion protein lysate.
    Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
    Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
    Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
    Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
    Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (<a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1602-7) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (<a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-7" style="font-weight: bold;text-decoration: underline;">ET1602-7</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1602-7) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation