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Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

Usd: 385

Specification

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Catalog# ET1603-1

Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Caveolin-1 aa 129-178 / 178.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 20 kDa

Positive Control

A549 cell lysate, A431 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse heart tissue lysate, rat lung tissue lysate, rat heart tissue lysate, A549, MCF-7, NIH/3T3, human liver tissue, human liver carcinoma tissue, human uterus tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

SZ02-01

RRID

AB_3069667

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:1,000

  • IHC-P

  • 1:1,000-1:10,000

Target

Function

Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.

Background References

1. Chen D et al. Glioma cell proliferation controlled by ERK activity-dependent surface expression of PDGFRA. PLoS One 9:e87281 (2014).

2. Olofsson A et al. Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways. MBio 5:e00979-14 (2014).

Sequence Similarity

Belongs to the caveolin family.

Tissue Specificity

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Post-translational Modification

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

Subcellular Location

Golgi apparatus membrane, Cell membrane, Membrane.

UNIPROT

SwissProt: Q03135 Human

SwissProt: P49817 Mouse

SwissProt: P41350 Rat

Synonyms

BSCL3 antibody

CAV antibody

CAV1 antibody

CAV1_HUMAN antibody

caveolae protein, 22 kD antibody

caveolin 1 alpha isoform antibody

caveolin 1 beta isoform antibody

Caveolin 1 caveolae protein 22kDa antibody

Caveolin-1 antibody

Caveolin1 antibody

Expand

Images

  • Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at different dilutions.<br /><br />Lane 1/2: A549 cell lysate<br />Lane 3/4: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at different dilutions.

    Lane 1/2: A549 cell lysate
    Lane 3/4: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (10 µg/Lane)<br />Lane 2: A431 cell lysate (10 µg/Lane)<br />Lane 3: Human lung tissue lysate (40 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat lung tissue lysate (40 µg/Lane)<br />Lane 7: Rat heart tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20/18 kDa<br /><br />Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (ET1603-1) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (10 µg/Lane)
    Lane 2: A431 cell lysate (10 µg/Lane)
    Lane 3: Human lung tissue lysate (40 µg/Lane)
    Lane 4: Mouse lung tissue lysate (40 µg/Lane)
    Lane 5: Mouse heart tissue lysate (40 µg/Lane)
    Lane 6: Rat lung tissue lysate (40 µg/Lane)
    Lane 7: Rat heart tissue lysate (40 µg/Lane)

    Predicted band size: 20 kDa
    Observed band size: 20/18 kDa

    Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Primary antibody dilution: 1/1,000<br />Antigen retrieval: ER2<br />Platform: Leica Biosystems BOND&reg; RX

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Primary antibody dilution: 1/1,000
    Antigen retrieval: ER2
    Platform: Leica Biosystems BOND® RX

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1603-1, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Uterus<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Uterus
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1603-1, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Heart<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/10,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Heart
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1603-1, 1/10,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Heart<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1603-1" style="font-weight: bold;text-decoration: underline;">ET1603-1</a>, 1/1,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Heart
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1603-1, 1/1,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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