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Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

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Specification

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Catalog# ET1603-22

Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr185 of Human Erk2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue

Molecular Weight

Predicted band size: 43/41 kDa

Positive Control

Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, A549, NIH/3T3, MCF-7, human lung carcinoma tissue, human kidney tissue, human gallbladder tissue.

Conjugation

unconjugated

Clone Number

SZ2-4

RRID

AB_3069673

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:50-1:100

  • IF-Tissue

  • 1:50-1:200

Target

Function

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Background References

1. Ruess DA et al. HDACi Valproic Acid (VPA) and Suberoylanilide Hydroxamic Acid (SAHA) Delay but Fail to Protect against Warm Hepatic Ischemia-Reperfusion Injury. PLoS One 11:e0161233 (2016).

2. Ahnstedt H et al. U0126 attenuates cerebral vasoconstriction and improves long-term neurologic outcome after stroke in female rats. J Cereb Blood Flow Metab 35:454-60 (2015).

Sequence Similarity

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Post-translational Modification

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

Subcellular Location

Cytoplasm, Nucleus, Membrane, Cell junction.

UNIPROT

SwissProt: P27361 Human

SwissProt: P28482 Human

SwissProt: P63085 Mouse

SwissProt: Q63844 Mouse

Synonyms

ERK 1 antibody

ERK 2 antibody

ERK-1 antibody

ERK-2 antibody

ERK1 antibody

erk1/2 antibody

ERK2 antibody

ERT1 antibody

ERT2 antibody

Extracellular signal regulated kinase 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Erk1 (T202)+Erk2 (T185) on different lysates with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: C6 treated with 200nM PMA for 30 minutes cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 41/43 kDa<br />Observed band size: 41/43 kDa<br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Erk1 (T202)+Erk2 (T185) on different lysates with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate
    Lane 5: C6 cell lysate
    Lane 6: C6 treated with 200nM PMA for 30 minutes cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 41/43 kDa
    Observed band size: 41/43 kDa
    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Erk1(T202)+Erk2(T185) on jurkat cell lysates.<br /><br />Lane 1: jurkat cells, whole cell lysate, 10ug/lane<br />Lane 2/3: jurkat cells treated with 200 ng/ml PMA for 35 minutes, whole cell lysate, 10ug/lane<br />Lane 4: jurkat cells treated with 200 ng/ml PMA for 35 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane<br /><br />All lanes :<br />Anti-Phospho-Erk1(T202)+Erk2(T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/500 dilution. Anti-Erk1+Erk2antibody (<a href="/products/ET1601-29" style="font-weight: bold;text-decoration: underline;">ET1601-29</a>) at 1/500 dilution. Anti-GAPDH antibody (<a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/200,000 dilution.<br /><br />Predicted band size: 41/43 kDa<br />Observed band size: 41/43 kDa<br /><br />Blocking and diluting buffer: 5% BSA.<br />Exposure time: Lan1/2 4 minutes; Lan3/4 3 minutes

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Erk1(T202)+Erk2(T185) on jurkat cell lysates.

    Lane 1: jurkat cells, whole cell lysate, 10ug/lane
    Lane 2/3: jurkat cells treated with 200 ng/ml PMA for 35 minutes, whole cell lysate, 10ug/lane
    Lane 4: jurkat cells treated with 200 ng/ml PMA for 35 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane

    All lanes :
    Anti-Phospho-Erk1(T202)+Erk2(T185) antibody (ET1603-22) at 1/500 dilution. Anti-Erk1+Erk2antibody (ET1601-29) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.

    Predicted band size: 41/43 kDa
    Observed band size: 41/43 kDa

    Blocking and diluting buffer: 5% BSA.
    Exposure time: Lan1/2 4 minutes; Lan3/4 3 minutes

  • ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Phospho-Erk1 (T202)+Erk2 (T185) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of Phospho-Erk1 (T202)+Erk2 (T185) was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Phospho-Erk1 (T202)+Erk2 (T185) was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1603-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-22" style="font-weight: bold;text-decoration: underline;">ET1603-22</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody (ET1603-22) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX."

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Citation

Products with the same target and pathway

metafield image

Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated