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CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]

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Specification

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Catalog# ET1702-46

CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CNPase Recombinant Rabbit Monoclonal Antibody [JF10-25]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CNPase aa 386-421 / 421.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 48 kDa

Positive Control

Mouse cerebellum tissue, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

JF10-25

RRID

AB_3070308

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:500

  • IF-Tissue

  • 1:100

Target

Function

2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase) is a membrane-bound enzyme that can link tubulin to membranes and may regulate cytoplasmic microtubule distribution. CNPase acts as a microtubule-associated protein by promoting microtubule assembly; this activity resides in the C-terminus of the enzyme. CNPase is firmly associated with tubulin from brain tissue and thyroid cells and can be found at high concentrations in central nervous system myelin and in the outer segments of photoreceptors in the retina. Acute lead intoxication leads to disturbances in CNPase activity and the morphology of myelin.

Background References

1. Chang S et al. Neuropsin Inactivation Has Protective Effects against Depressive-Like Behaviours and Memory Impairment Induced by Chronic Stress. PLoS Genet 12:e1006356 (2016).

2. Kuzdas-Wood D et al. Involvement of Peripheral Nerves in the Transgenic PLP-a-Syn Model of Multiple System Atrophy: Extending the Phenotype. PLoS One 10:e0136575 (2015).

Sequence Similarity

Belongs to the 2H phosphoesterase superfamily. CNPase family.

Subcellular Location

Membrane, Melanosome.

UNIPROT

SwissProt: P09543 Human

SwissProt: P16330 Mouse

SwissProt: P13233 Rat

Synonyms

2' antibody

2'3' cyclic nucleotide 3' phosphodiesterase antibody

3'-cyclic-nucleotide 3'-phosphodiesterase antibody

CN37_HUMAN antibody

CNP 1 antibody

CNP antibody

CNP1 antibody

CNPase antibody

Images

  • Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling CNPase with Rabbit anti-CNPase antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling CNPase with Rabbit anti-CNPase antibody (ET1702-46) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-46, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CNPase on different lysates with Rabbit anti-CNPase antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate<br />Lane 2: Mouse liver tissue lysate (low expression)<br />Lane 3: Rat cerebellum tissue lysate<br />Lane 4: Rat liver tissue lysate (low expression)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CNPase on different lysates with Rabbit anti-CNPase antibody (ET1702-46) at 1/2,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate
    Lane 2: Mouse liver tissue lysate (low expression)
    Lane 3: Rat cerebellum tissue lysate
    Lane 4: Rat liver tissue lysate (low expression)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 14 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CNPase antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CNPase antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-46" style="font-weight: bold;text-decoration: underline;">ET1702-46</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CNPase antibody (ET1702-46) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-46) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation