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Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10]

Usd: 385

Specification

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Catalog# ET1703-17

Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10]

Application

  • WB

  • FC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal human Interferon gamma.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 19 kDa

Positive Control

Human IFN-gamma recombinant protein, Jurkat.

Conjugation

unconjugated

Clone Number

JM10-10

RRID

AB_3070374

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

Target

Function

Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. In humans, the IFN-γ protein is encoded by the IFNG gene. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoan infections. IFN-γ is an important activator of macrophages and inducer of major histocompatibility complex class II molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer cells (NK) and natural killer T cells (NKT) as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops as part of the adaptive immune response. IFN-γ is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s.

Background References

1. Kaewkangsadan V et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016).

2. Srivastava SP et al. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis. Sci Rep 6:29884 (2016).

Sequence Similarity

Belongs to the type II (or gamma) interferon family.

Tissue Specificity

Released primarily from activated T lymphocytes.

Post-translational Modification

Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01579 Human

Synonyms

IFG antibody

IFI antibody

IFN gamma antibody

IFN, immune antibody

IFN-gamma antibody

IFNG antibody

IFNG_HUMAN antibody

Immune interferon antibody

Interferon gamma antibody

Images

  • Western blot analysis of Interferon gamma on different lysates with Rabbit anti-Interferon gamma antibody (<a href="/products/ET1703-17" style="font-weight: bold;text-decoration: underline;">ET1703-17</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane<br />Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane<br />Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-17" style="font-weight: bold;text-decoration: underline;">ET1703-17</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Interferon gamma on different lysates with Rabbit anti-Interferon gamma antibody (ET1703-17) at 1/1,000 dilution.

    Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane
    Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane
    Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-17) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1703-17" style="font-weight: bold;text-decoration: underline;">ET1703-17</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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