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Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]

Usd: 385

Specification

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Catalog# ET1703-24

Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Haptoglobin aa 357-406 / 406.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Human serum lysate, Human plasma lysate, human liver tissue, human lung tissue, HepG2.

Conjugation

unconjugated

Clone Number

JM10-79

RRID

AB_3070381

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

Target

Function

Haptoglobin (abbreviated as Hp) is the protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds with high affinity to free hemoglobin released from erythrocytes, and thereby inhibits its deleterious oxidative activity. Hemoglobin that has been released into the blood plasma by damaged red blood cells has harmful effects. The HP gene encodes a preproprotein that is processed to yield both alpha and beta chains, which subsequently combines as a tetramer to produce haptoglobin. Haptoglobin functions to bind the free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. The cellular receptor target of Hp is the monocyte/macrophage scavenger receptor, CD163. Following Hb-Hp binding to CD163, cellular internalization of the complex leads to globin and heme metabolism, which is followed by adaptive changes in antioxidant and iron metabolism pathways and macrophage phenotype polarization.

Background References

1. Abdullah M & Goldmann T Pulmonary haptoglobin (pHp) is part of the surfactant system in the human lung. Diagn Pathol 7:158 (2012).

2. Tomazic PV et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013).

Sequence Similarity

Belongs to the peptidase S1 family.

Tissue Specificity

Expressed by the liver and secreted in plasma.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P00738 Human

Synonyms

Binding peptide antibody

BP antibody

Haptoglobin alpha chain antibody

Haptoglobin alpha(1S) beta antibody

Haptoglobin alpha(2FS) beta antibody

Haptoglobin beta chain antibody

Haptoglobin, alpha polypeptide antibody

Haptoglobin, beta polypeptide antibody

HP antibody

HP2 ALPHA2 antibody

Expand

Images

  • Western blot analysis of Haptoglobin on different lysates with Rabbit anti-Haptoglobin antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human serum lysate<br />Lane 2: Human plasma lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Haptoglobin on different lysates with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution.

    Lane 1: Human serum lysate
    Lane 2: Human plasma lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Haptoglobin antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Haptoglobin antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining of Haptoglobin in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1703-24" style="font-weight: bold;text-decoration: underline;">ET1703-24</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Haptoglobin in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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