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CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]

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Specification

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Catalog# ET1703-48

CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CYP2E1 aa 71-114 / 439.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P, mIHC

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Human liver tissue lysates, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

JM10-85

RRID

AB_3070404

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • mIHC

  • 1:2,000

Target

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).

Background References

1. Gi M et al. Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-Dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. Toxicol Sci 145:196-208 (2015).

2. Boussadia B et al. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 281C:124-134 (2014).

Sequence Similarity

Belongs to the cytochrome P450 family.

Subcellular Location

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.

UNIPROT

SwissProt: P05181 Human

SwissProt: Q05421 Mouse

SwissProt: P05182 Rat

Synonyms

4-nitrophenol 2-hydroxylase antibody

CP2E1_HUMAN antibody

CPE1 antibody

CYP2E antibody

Cyp2e1 antibody

CYPIIE1 antibody

Cytochrome P450 2E1 antibody

Cytochrome P450 isozyme 3A antibody

Cytochrome P450 LM3A antibody

Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody

Expand

Images

  • Western blot analysis of CYP2E1 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CYP2E1 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CYP2E1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Rat liver tissue lysate

    Western blot analysis of CYP2E1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CYP2E1 antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CYP2E1 antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CYP2E1 antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-48" style="font-weight: bold;text-decoration: underline;">ET1703-48</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: liver<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation