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Peroxiredoxin 6 Recombinant Rabbit Monoclonal Antibody [JM11-13]

Usd: 385

Specification

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Catalog# ET1703-62

Peroxiredoxin 6 Recombinant Rabbit Monoclonal Antibody [JM11-13]

Application

  • WB

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Peroxiredoxin 6 Recombinant Rabbit Monoclonal Antibody [JM11-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Peroxiredoxin 6 aa 175-224 / 224.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 25 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

JM11-13

RRID

AB_3073469

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

Target

Function

Peroxiredoxin-6 is a protein that in humans is encoded by the PRDX6 gene. It is a member of the peroxiredoxin family of antioxidant enzymes. Peroxiredoxin 6 is widely distributed in several organs, especially the lungs. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Background References

1. Wang Y et al. Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis. Cancer Sci 107:250-7 (2016).

2. Trevisan R et al. Zinc causes acute impairment of glutathione metabolism followed by coordinated antioxidant defenses amplification in gills of brown mussels Perna perna. Comp Biochem Physiol C Toxicol Pharmacol 159:22-30 (2014).

Sequence Similarity

Belongs to the peroxiredoxin family. Prx6 subfamily.

Post-translational Modification

Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.; Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme.

Subcellular Location

Cytoplasm. Lysosome.

UNIPROT

SwissProt: P30041 Human

SwissProt: O08709 Mouse

SwissProt: O35244 Rat

Synonyms

1 Cys antibody

1 Cys peroxiredoxin antibody

1 Cys PRX antibody

1 cysPrx antibody

1-Cys peroxiredoxin antibody

1-Cys PRX antibody

24 kDa protein antibody

9430088D19Rik antibody

AA690119 antibody

Acidic calcium independent phospholipase A2 antibody

Expand

Images

  • Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (<a href="/products/ET1703-62" style="font-weight: bold;text-decoration: underline;">ET1703-62</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat lung tissue lysate (40 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-62" style="font-weight: bold;text-decoration: underline;">ET1703-62</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: Mouse lung tissue lysate (40 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat lung tissue lysate (40 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 27 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (<a href="/products/ET1703-62" style="font-weight: bold;text-decoration: underline;">ET1703-62</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-62" style="font-weight: bold;text-decoration: underline;">ET1703-62</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 27 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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