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Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86]

Usd: 385

Specification

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Catalog# ET7108-01

Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86]

Application

  • WB

  • IHC-P

  • FC

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Glucose 6 phosphate isomerase aa 1-128 / 558.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 63 kDa

Positive Control

A549 cell lysates, HepG2 cell lysates, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue, A549.

Conjugation

unconjugated

Clone Number

JG33-86

RRID

AB_3070804

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.

Background References

1. Hong Z et al. Esculetin inhibits liver cancer by targeting glucose-6-phosphate isomerase mediated glycolysis. Biomed Pharmacother. 2025 Jul

2. Gruda Sussman R et al. Glucose 6 Phosphate Isomerase Deficiency, a Rare Hemolytic Anemia Misdiagnosed as Hereditary Spherocytosis. J Pediatr Hematol Oncol. 2023 Jan

Sequence Similarity

Belongs to the GPI family.

Post-translational Modification

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.; ISGylated.

Subcellular Location

Cytoplasm. Secreted.

UNIPROT

SwissProt: P06744 Human

Synonyms

AMF antibody

Aurocrine motility factor antibody

Autocrine motility factor antibody

DKFZp686C13233 antibody

EC 5.3.1.9 antibody

G6PI_HUMAN antibody

Glucose phosphate isomerase antibody

Glucose-6-phosphate isomerase antibody

GNPI antibody

GPI antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Glucose 6 phosphate isomerase on different lysates with Rabbit anti-Glucose 6 phosphate isomerase antibody (<a href="/products/ET7108-01" style="font-weight: bold;text-decoration: underline;">ET7108-01</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Glucose 6 phosphate isomerase KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-01" style="font-weight: bold;text-decoration: underline;">ET7108-01</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Glucose 6 phosphate isomerase on different lysates with Rabbit anti-Glucose 6 phosphate isomerase antibody (ET7108-01) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Glucose 6 phosphate isomerase KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 54 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-01) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Glucose 6 phosphate isomerase on A549 cell lysates and HepG2 cell lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.

    Western blot analysis of Glucose 6 phosphate isomerase on A549 cell lysates and HepG2 cell lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.

  • ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation