Skip to content

IL-5 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# HA500081

IL-5 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • ELISA

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL-5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human IL-5 aa 60-110.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, ELISA

Molecular Weight

Predicted band size: 15 kDa

Positive Control

LOVO cell lysates, LOVO cells.

Conjugation

unconjugated

RRID

AB_3071180

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • ELISA

  • 1:5,000-1:10,000

Target

Function

Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells.Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion - primarily IgA. It is also a key mediator in eosinophil activation.IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer.

Background References

1. Minamitake Y. et. al. Structure of recombinant human interleukin 5 produced by Chinese hamster ovary cells. J. Biochem. 107:292-297(1990).

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05113 Human

Synonyms

B-cell differentiation factor I antibody

Colony stimulating factor antibody

EDF antibody

Eosinophil differentiation factor antibody

IL-5 antibody

IL5 antibody

IL5_HUMAN antibody

Interleukin 5 (colony stimulating factor, eosinophil) antibody

Interleukin 5 antibody

Interleukin-5 antibody

Expand

Images

  • Western blot analysis of IL-5 on LOVO cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500081" style="font-weight: bold;text-decoration: underline;">HA500081</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IL-5 on LOVO cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500081, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • ICC staining of IL-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500081" style="font-weight: bold;text-decoration: underline;">HA500081</a>, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of IL-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500081, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation