Adipose Triglyceride Lipase Rabbit Polyclonal Antibody
Catalog# HA500301
Adipose Triglyceride Lipase Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
Adipose Triglyceride Lipase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Adipose Triglyceride Lipase aa 450-504 / 504 (Lumenal).
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
55 kDa
Positive Control
293 cell lysate, HepG2 cell lysate, A431 cell lysate, human pancreas tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:1,000-1:2,000
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IHC-P
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1:100-1:500
Target
Function
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone. Also has acylglycerol transacylase activity. Acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
Background References
1. Trites MJ. et. al. The role of adipose triglyceride lipase in lipid and glucose homeostasis: lessons from transgenic mice. Lipids Health Dis. 2019 Nov
2. Schreiber R. et. al. Of mice and men: The physiological role of adipose triglyceride lipase (ATGL). Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Jun
Subcellular Location
Cell membrane, Lipid droplet.
UNIPROT
Synonyms
1110001C14Rik antibody
Adipose triglyceride lipase antibody
ATGL antibody
ATGL DESNUTRIN antibody
Calcium independent phospholipase A2 antibody
Calcium-independent phospholipase A2 antibody
Desnutrin antibody
EC 3.1.1.3 antibody
FP17548 antibody
IPLA2 zeta antibody
ExpandImages
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Western blot analysis of Adipose Triglyceride Lipase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500301, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A431 cell lysate -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Adipose Triglyceride Lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500301, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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