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Adipose Triglyceride Lipase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500301

Adipose Triglyceride Lipase Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Adipose Triglyceride Lipase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Adipose Triglyceride Lipase aa 450-504 / 504 (Lumenal).

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

55 kDa

Positive Control

293 cell lysate, HepG2 cell lysate, A431 cell lysate, human pancreas tissue.

Conjugation

unconjugated

RRID

AB_3071396

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:100-1:500

Target

Function

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone. Also has acylglycerol transacylase activity. Acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.

Background References

1. Trites MJ. et. al. The role of adipose triglyceride lipase in lipid and glucose homeostasis: lessons from transgenic mice. Lipids Health Dis. 2019 Nov

2. Schreiber R. et. al. Of mice and men: The physiological role of adipose triglyceride lipase (ATGL). Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Jun

Subcellular Location

Cell membrane, Lipid droplet.

UNIPROT

SwissProt: Q96AD5 Human

Synonyms

1110001C14Rik antibody

Adipose triglyceride lipase antibody

ATGL antibody

ATGL DESNUTRIN antibody

Calcium independent phospholipase A2 antibody

Calcium-independent phospholipase A2 antibody

Desnutrin antibody

EC 3.1.1.3 antibody

FP17548 antibody

IPLA2 zeta antibody

Expand

Images

  • Western blot analysis of Adipose Triglyceride Lipase on HEK-293 cell lysate with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA500301" style="font-weight: bold;text-decoration: underline;">HA500301</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500301" style="font-weight: bold;text-decoration: underline;">HA500301</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 55.3 kDa<br />Observed band size: 55 kDa

    Western blot analysis of Adipose Triglyceride Lipase on HEK-293 cell lysate with Rabbit anti-Adipose Triglyceride Lipase antibody (HA500301) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500301, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55.3 kDa
    Observed band size: 55 kDa

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Adipose Triglyceride Lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500301" style="font-weight: bold;text-decoration: underline;">HA500301</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Adipose Triglyceride Lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500301, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

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Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49]

Application: WB,IHC-P,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated