PERK Rabbit Polyclonal Antibody (HA500326)
Catalog# HA500326
Rabbit Polyclonal to PERK
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WB
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Human
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 125 kDa
Observed band size: 130 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500326) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PERK Rabbit Polyclonal Antibody (HA500326)
Immunogen
Synthetic peptide within human perk aa 1,067-1,116/1,116.
Host
Rabbit
Positive Control
MCF-7 cell lysate, 293 cell lysate, HepG2 cell lysate, Hela cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 125 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PERK
SYNONYMS
DKFZp781H1925 antibody;E2AK3_HUMAN antibody;EC 2.7.11.1 antibody;Eif2ak3 antibody;Eukaryotic translation initiation factor 2 alpha kinase 3 antibody;Eukaryotic translation initiation factor 2-alpha kinase 3 antibody;Heme regulated EIF2 alpha kinase antibody;HRI antibody;HsPEK antibody;Pancreatic eIF2 alpha kinase antibody;Pancreatic eIF2-alpha kinase antibody;PEK antibody;PRKR like endoplasmic reticulum kinase antibody;PRKR-like endoplasmic reticulum kinase antibody;WRS antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
CITATIONS
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Feng Yu,et al
Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib
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WB
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Human
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Citation
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