Frizzled 7 Rabbit Polyclonal Antibody (HA500433)
Catalog# HA500433
Rabbit Polyclonal to Frizzled 7
Tested for
-
WB
Reacts with
-
Human
Western blot analysis of Frizzled 7 on different lysates with Rabbit anti-Frizzled 7 antibody (HA500433) at 1/500 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Human skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 64 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500433) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Human skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 64 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500433) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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