Frizzled 7 Rabbit Polyclonal Antibody
Catalog# HA500433
Frizzled 7 Rabbit Polyclonal Antibody
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WB
-
Human
-
unconjugated
Overview
Product Name
Frizzled 7 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Frizzled 7 aa 525-574 / 574.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 64 kDa
Positive Control
HepG2 cell lysate, 293 cell lysate, 293T cell lysate, human skeletal muscle tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Frizzled-7 (Fd-7) is a protein that in humans is encoded by the FZD7 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. Fz-7 has been shown to interact with DLG4.
Background References
1. Wang Y et al. Frizzled-7 Identifies Platinum-Tolerant Ovarian Cancer Cells Susceptible to Ferroptosis. Cancer Res. 2021 Jan
2. He W et al. Activation of Frizzled-7 attenuates blood-brain barrier disruption through Dvl/β-catenin/WISP1 signaling pathway after intracerebral hemorrhage in mice. Fluids Barriers CNS. 2021 Sep
Subcellular Location
Cell membrane, Endosome membrane.
UNIPROT
Synonyms
Frizzled 7, seven transmembrane spanning receptor antibody
frizzled class receptor 7 antibody
Frizzled drosophila homolog of 7 antibody
Frizzled family receptor 7 antibody
Frizzled homolog 7 antibody
Frizzled-7 antibody
Frizzled7 antibody
Fz 7 antibody
Fz-7 antibody
Fz7 antibody
ExpandImages
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Western blot analysis of Frizzled 7 on different lysates with Rabbit anti-Frizzled 7 antibody (HA500433) at 1/1000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Predicted band size: 64 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500433) at 1/1000 dilution was used in primary antibody dilution buffer (K1803), overnight at 4 ℃.
Secondary antibody: Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:500,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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