TGF beta 1 Rabbit Polyclonal Antibody
Catalog# HA500496
TGF beta 1 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
TGF beta 1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human TGF beta 1 aa 20-210.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 44 kDa
Positive Control
PC-3 cell lysate, A549 cell lysate, U87 cell lysate, Hela cell lysate, K562 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Background References
1. Robertson I.B., et al. Regulation of the bioavailability of TGF-beta and TGF-beta-related proteins. Cold Spring Harb. Perspect. Biol. 8:0-0(2016).
2. Miyazono K., et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. EMBO J. 10:1091-1101(1991) .
Subcellular Location
Extracellular matrix, Secreted.
UNIPROT
Synonyms
Cartilage-inducing factor antibody
CED antibody
Differentiation inhibiting factor antibody
DPD1 antibody
LAP antibody
Latency-associated peptide antibody
Prepro transforming growth factor beta 1 antibody
TGF beta 1 antibody
TGF beta antibody
TGF beta 1 protein antibody
ExpandImages
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Western blot analysis of TGF beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500496, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: U87 cell lysate
Lane 4: Hela cell lysate
Lane 5: K562 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Chrysin inhibits hypertrophic scar formation through TGF-β/Smad signaling pathways
Journal: Journal Of Molecular Histology
DOI: 10.1007/s10735-025-10576-3
IF: 2.2
Application: WB
Reactivity: Human
Publish date: 2025 Sept
-
The Kinesin Eg5 Inhibitor K858 Enhances Radiosensitivity in Esophageal Squamous Cell Carcinoma and Affects the Expression of Epithelial-mesenchymal Transition Related Markers: In vitro and In vivo Studies
Journal: Anti-Cancer Agents In Medicinal Chemistry
DOI: 10.2174/0118715206373782250211104402
IF: 3
Application: WB
Reactivity: Human
Publish date: 2025 Feb
-
Dietary biotin facilitates collagen deposition and myofiber growth and development, improving muscle chewiness and hardness in grass carp (Ctenopharyngodon idella)
Journal: Food Bioscience
DOI: 10.1016/j.fbio.2025.107461
IF: 5.9
Application: WB
Reactivity: Fish
Publish date: 2025 Aug
-
Hongjingtian alleviates arsenic-induced hepatocyte injury by inhibiting oxidative stress and endoplasmic reticulum stress
Journal:
DOI: 10.1080/10286020.2025.2481290
IF:
Application: WB
Reactivity: Fish
Publish date: 2025 Apr
-
PEEP application during mechanical ventilation contributes to fibrosis in the diaphragm
Journal: Respiratory Research
DOI:
IF: 7.162
Application: WB
Reactivity: Rabbit
Publish date: 2023 Feb
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Conjugate: unconjugated
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