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TATA binding protein TBP Rabbit Polyclonal Antibody

Usd: 295

Specification

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Catalog# HA500518

TATA binding protein TBP Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TATA binding protein TBP Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human TBP aa 140-339 / 339.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 38 kDa

Positive Control

Hela cell lysate, HCT116 cell lysate, 293T cell lysate, HepG2 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human bladder carcinoma tissue, human testis tissue, human colon tissue, mouse stomach tissue.

Conjugation

unconjugated

RRID

AB_3071602

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:2,000

Target

Function

The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. This DNA sequence is found about 30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP is a member of a small gene family of TBP-related factors. The first TBP-related factor (TRF/TRF1) was identified in the fruit fly Drosophila, but appears to be fly or insect-specific. Subsequently TBPL1/TRF2 was found in the genomes of many metazoans, whereas vertebrate genomes encode a third vertebrate family member, TBPL2/TRF3. In specific cell types or on specific promoters TBP can be replaced by one of these TBP-related factors, some of which interact with the TATA box similarly to TBP.

Background References

1. Wang H et al. Structures and implications of TBP-nucleosome complexes. Proc Natl Acad Sci U S A. 2021 Jul

2. Magri S et al. Digenic inheritance of STUB1 variants and TBP polyglutamine expansions explains the incomplete penetrance of SCA17 and SCA48. Genet Med. 2022 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P20226 Human

SwissProt: P29037 Mouse

Entrez Gene: 117526 Rat

Synonyms

GTF2D antibody

GTF2D1 antibody

HDL4 antibody

MGC117320 antibody

MGC126054 antibody

MGC126055 antibody

SCA17 antibody

TATA binding factor antibody

TATA box factor antibody

TATA sequence binding protein antibody

Expand

Images

  • Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate (20 µg/Lane)<br />Lane 2: HCT116 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: MCF-7 cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: C2C12 cell lysate (20 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 9: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 10: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 11: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 40/36 kDa<br /><br />Exposure time: 3 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/1,000 dilution.

    Lane 1: Hela cell lysate (20 µg/Lane)
    Lane 2: HCT116 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: MCF-7 cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: C2C12 cell lysate (20 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 9: RAW264.7 cell lysate (20 µg/Lane)
    Lane 10: Mouse testis tissue lysate (40 µg/Lane)
    Lane 11: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 40/36 kDa

    Exposure time: 3 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500518) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TATA binding protein TBP antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TATA binding protein TBP antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TATA binding protein TBP antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TATA binding protein TBP antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500518" style="font-weight: bold;text-decoration: underline;">HA500518</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TATA binding protein TBP antibody (HA500518) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500518) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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