Dicer Rabbit Polyclonal Antibody
Catalog# HA500522
Dicer Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Dicer Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human aa 701-1,100 / 1,922.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 219 kDa
Positive Control
MCF-7 cell lysate, 293 cell lysate, Daudi cell lysate, MCF-7, C2C12.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
|
||
| Mouse |
|
||
| Rat |
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000
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IF-Cell
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10ug/mL
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FC
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1ug/mL
Target
Function
Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. Being part of the RNase III family, Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short double-stranded RNA fragments called small interfering RNA and microRNA, respectively. These fragments are approximately 20–25 base pairs long with a two-base overhang on the 3′-end. Dicer facilitates the activation of the RNA-induced silencing complex (RISC), which is essential for RNA interference. RISC has a catalytic component Argonaute, which is an endonuclease capable of degrading messenger RNA (mRNA).
Background References
1. Poirier EZ et al. An isoform of Dicer protects mammalian stem cells against multiple RNA viruses. Science. 2021 Jul
2. Wang Q et al. Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation. Science. 2021 Nov
Subcellular Location
Cytoplasm, perinuclear region.
Synonyms
DCR antibody
DCR1 antibody
Dicer 1 ribonuclease III antibody
Dicer 1 ribonuclease type III antibody
Dicer antibody
DICER_HUMAN antibody
DICER1 antibody
Double-strand-specific ribonuclease antibody
Endoribonuclease Dicer antibody
Helicase MOI antibody
ExpandImages
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Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA500522) at 1/1,000 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 219 kDa
Observed band size: 250 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF-7 cells labeling Dicer with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of C2C12 cells labeling Dicer.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500522, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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