SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A4G9] - BSA and Azide free (Detector)
Catalog# HA600005
SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A4G9] - BSA and Azide free (Detector)
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WB
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ELISA(Det)
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SARS-CoV-2
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31,250-4,000,000 pg/ml
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unconjugated
Overview
Product Name
SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A4G9] - BSA and Azide free (Detector)
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419.
Species Reactivity
SARS-CoV-2
Validated Applications
WB, ELISA(Det)
Molecular Weight
46 kDa
Positive Control
SARS-CoV-2 nucleocapsid protein.
Conjugation
unconjugated
Clone Number
A4G9
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH 7.4).
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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0.5-2ug/ml
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ELISA(Det)
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10ng/ml
Target
Function
The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Background References
1. Fan Wu. et. al. A new coronavirus associated with human respiratory disease in China. Nature. 2020 Mar;579(7798):265-269.
Subcellular Location
Virion.
Synonyms
2019-nCoV nucleocapsid protein antibody
N antibody
NC antibody
Nucleoprotein antibody
Protein N antibody
Images
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The binding activity of HA600005 with Recombinant SARS-CoV-2 Nucleocapsid protein.
Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600005) for 1 hour at 25℃. The EC50 of HA600005 is 47.22 ng/ml. -
This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Clone: # A4G9-HRP). In a sandwich ELISA, it can be used as Detect antibody when paired with (Cat: # HA600007).
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Sandwich ELISA analysis of SARS-CoV-2 Nucleocapsid protein matched pair antibodies
Capture: HA600008, SARS-CoV-2 Nucleocapsid protein Rabbit mAb [A5B8]
Detector: HA600005, SARS-CoV-2 Nucleocapsid protein Rabbit mAb [A4G9]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA600008) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant SARS-CoV-2 Nucleocapsid protein protein (HAg2002) starting from 4,000,000 pg/ml to 0 pg/ml and detect antibody (HA600005, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"