SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A5B10] - BSA and Azide free (Capture)
Overview
Product Name
SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A5B10] - BSA and Azide free (Capture)
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419.
Species Reactivity
SARS-CoV-2
Validated Applications
WB, ELISA(Cap)
Molecular Weight
46 kDa
Positive Control
SARS-CoV-2 nucleocapsid protein.
Conjugation
unconjugated
Clone Number
A5B10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH 7.4).
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
0.5-2ug/ml
-
ELISA(Cap)
-
10ng/ml
Target
Function
The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Background References
1. Fan Wu. et. al. A new coronavirus associated with human respiratory disease in China. Nature. 2020 Mar;579(7798):265-269.
Subcellular Location
Virion.
Synonyms
2019-nCoV nucleocapsid protein antibody
N antibody
NC antibody
Nucleoprotein antibody
Protein N antibody
Images
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The binding activity of HA600007 with Recombinant SARS-CoV-2 Nucleocapsid protein.
Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600007) for 1 hour at 25℃. The EC50 of HA600007 is 11.82 ng/ml. -
This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Cat: # HA600007). In a sandwich ELISA, it can be used as Capture antibody when paired with (Clone: # A4G9-HRP).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"