CD151 Mouse Monoclonal Antibody [A6G11]
Catalog# HA600059
CD151 Mouse Monoclonal Antibody [A6G11]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
CD151 Mouse Monoclonal Antibody [A6G11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD151 aa 104-253.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 28 kDa
Positive Control
HepG2 cell lysate, A431, A549, human kidney tissue, mouse liver tissue.
Conjugation
unconjugated
Clone Number
A6G11
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:100
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IHC-P
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1:500
Target
Function
CD151 molecule (Raph blood group), also known as CD151 (Cluster of Differentiation 151), is a human gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. Abnormalities in CD151 have been implicated in a form of epidermolysis bullosa.
Background References
1. Peng D. et. al. Key Role of CD151-integrin Complex in Lung Cancer Metastasis and Mechanisms Involved. Curr Med Sci. 2020 Dec
2. Han R. et. al. Integrin-associated CD151 is a suppressor of prostate cancer progression. Am J Transl Res. 2020 Apr
Subcellular Location
Membrane.
Synonyms
CD151 antibody
CD151 antigen antibody
CD151 molecule (Raph blood group) antibody
CD151 molecule antibody
CD151_HUMAN antibody
GP27 antibody
hemidesmosomal tetraspanin CD151 antibody
Membrane glycoprotein SFA-1 antibody
MER2 antibody
PETA-3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD151 on different lysates with Mouse anti-CD151 antibody (HA600059) at 1/1,000 dilution.
Lane 1: Daudi cell lysate (negative)
Lane 2: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600059) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling CD151 with Mouse anti-CD151 antibody (HA600059) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD151 antibody (HA600059) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of A549 cells labeling CD151 with Mouse anti-CD151 antibody (HA600059) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD151 antibody (HA600059) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD151 antibody (HA600059) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600059) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-CD151 antibody (HA600059) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600059) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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