p21 Mouse Monoclonal Antibody [A8C12]
Catalog# HA601017
p21 Mouse Monoclonal Antibody [A8C12]
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IHC-P
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Human
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unconjugated
Overview
Product Name
p21 Mouse Monoclonal Antibody [A8C12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within full length of human p21.
Species Reactivity
Human
Validated Applications
IHC-P
Molecular Weight
Predicted band size: 18 kDa
Positive Control
Human cervical well-differentiated squamous cell carcinoma tissue, human thyroid carcinoma tissue.
Conjugation
unconjugated
Clone Number
A8C12
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:400
Target
Function
p21WAF1, also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a protein that in humans is encoded by the CDKN1A gene located on chromosome 6 (6p21.2). May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. p21 is expressed in all adult human tissues. In tumors, the expression of p21 has been studied by immunohistochemical methods in a wide range of human tumors, such as gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. The expression of p21 is associated with favorable prognosis in various tumors.
Background References
1. Kaya Z. et. al. CDKN1A (p21 gene) polymorphisms correlates with age in esophageal cancer. Mol Biol Rep. 2022 Jan
2. Kreis NN. et. al. The Multifaceted p21 (Cip1/Waf1/CDKN1A) in Cell Differentiation, Migration and Cancer Therapy. Cancers (Basel). 2019 Aug
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
CAP20 antibody
CDK-interacting protein 1 antibody
CDKI antibody
CDKN1 antibody
Cdkn1a antibody
CDN1A_HUMAN antibody
CIP1 antibody
Cyclin Dependent Kinase Inhibitor 1A antibody
Cyclin-dependent kinase inhibitor 1 antibody
Cyclin-dependent kinase inhibitor 1A (P21) antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human cervical well-differentiated squamous cell carcinoma tissue with Mouse anti-p21 antibody (HA601017) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601017) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-p21 antibody (HA601017) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601017) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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