TMED9 Mouse Monoclonal Antibody [A9A7]
Catalog# HA601086
TMED9 Mouse Monoclonal Antibody [A9A7]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TMED9 Mouse Monoclonal Antibody [A9A7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human TMED9 aa 31-220 / 235.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 27 kDa
Positive Control
Hela cell lysate, THP-1 cell lysate, A549 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, LO2 cell lysate, human liver tissue lysate, rat liver tissue lysate, mouse liver tissue.
Conjugation
unconjugated
Clone Number
A9A7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000-1:10,000
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IHC-P
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1:1,000
Target
Function
Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB.
Background References
1. Li S et al. A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. Cell Res. 2022 Feb
2. Puri C et al. TMED9-SEC12, an important "contact" for autophagy. Cell Res. 2022 Feb
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus, Endoplasmic reticulum-Golgi intermediate compartment membrane.
Synonyms
Transmembrane emp24 domain-containing protein 9
GMP25
Glycoprotein 25L2
p24 family protein alpha-2 (p24alpha2)
p25
TMED9
GP25L2
Images
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-TMED9 antibody (HA601086) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (HA601086) at 1/1,000 dilution.
Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate
Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 4: MCF-7 (Human breast cancer cell) cell lysate
Lane 5: HepG2 (Human hepatocellular carcinoma cell) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 8: Mouse Liver tissue lysate
Lane 9: Rat Liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 43 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601086, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 27 kDa
Observed band size: 27 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"