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Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]

Usd: 350

Specification

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Catalog# HA601369

Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

  • IP

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human Calretinin aa 1-200.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue, IP

Molecular Weight

Predicted band size: 32 kDa

Positive Control

Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH08-29

Reactivity Data

WB IHC-P IHC-Fr IF-Tissue IP
Mouse
Tested Tested
Tested Tested
Not Recommended
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:500

  • IP

  • 1-2μg/sample

Target

Function

Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. Calretinin is abundantly expressed in neurons including retina (which gave it the name) and cortical interneurons. Expression was found in different neurons than that of the similar vitamin D-dependent calcium-binding protein, calbindin-28kDa. Calretinin has an important role as a modulator of neuronal excitability including the induction of long-term potentiation. Loss of expression of calretinin in hippocampal interneurons has been suggested to be relevant in temporal lobe epilepsy. It is expressed in a number of other locations including hair follicles.

Background References

1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).

2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).

Subcellular Location

Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.

UNIPROT

SwissProt: Q08331 Mouse

SwissProt: P47728 Rat

Synonyms

29 kDa calbindin antibody

CAB 29 antibody

CAB29 antibody

CAL 2 antibody

CAL2 antibody

CALB 2 antibody

CALB2 antibody

CALB2_HUMAN antibody

Calbindin 2 29kDa antibody

Calbindin 2 antibody

Expand

Images

  • Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse colon tissue lysate (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat colon tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 32 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (HA601369) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse colon tissue lysate (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)
    Lane 4: Rat colon tissue lysate (20 µg/Lane)

    Predicted band size: 32 kDa
    Observed band size: 29 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601369) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Retina<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Retina

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Calretinin antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hippocampus <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Mouse

    Site: Hippocampus

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a> at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a> IP in mouse brain tissue lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA601369" style="font-weight: bold;text-decoration: underline;">HA601369</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: Primary Antibody Dilution<br />Exposure time: 40 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA601369 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601369 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA601369 IP in mouse brain tissue lysate
    Lane 3: Mouse IgG instead of HA601369 in mouse brain tissue lysate

    Blocking/Dilution buffer: Primary Antibody Dilution
    Exposure time: 40 seconds; ECL: K1801

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