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GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)

Usd: 315

Specification

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Catalog# HA601389

GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Human

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein.

Product Specificity

This Rat Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (HA722750). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Species Reactivity

Mouse, Rat (Predicted: Human, Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 65 kDa

Positive Control

Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-80

Reactivity Data

IHC-Fr IHC-P WB
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Human
Published
Published
Published
Cynomolgus Monkey
Published
Published
Published
Pig
Published
Published
Published

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:2,000

  • IHC-P

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Background References

1. Budhram A et al. Clinical spectrum of high-titre GAD65 antibodies. J Neurol Neurosurg Psychiatry. 2021 Feb

2. Budhram A et al. Positive Predictive Value of Anti-GAD65 ELISA Cut-Offs for Neurological Autoimmunity. Can J Neurol Sci. 2023 Sep

Subcellular Location

Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane.

UNIPROT

SwissProt: P48320 Mouse

SwissProt: Q05683 Rat

Synonyms

65 kDa glutamic acid decarboxylase antibody

DCE 2 antibody

DCE2 antibody

DCE2_HUMAN antibody

GAD 2 antibody

GAD 65 antibody

GAD-2 antibody

GAD-65 antibody

Gad2 antibody

Glutamate decarboxylase 2 (pancreas) antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 2,000 (GAD65, <a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>, green); 1:2,000 (EAAT1, <a href="/products/ET1704-54" style="font-weight: bold;text-decoration: underline;">ET1704-54</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 2,000 (GAD65, <a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>, green); 1:2,000 (EAAT1, <a href="/products/ET1704-54" style="font-weight: bold;text-decoration: underline;">ET1704-54</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-GAD65 antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of GAD65 on different lysates with Rat anti-GAD65 antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601389" style="font-weight: bold;text-decoration: underline;">HA601389</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GAD65 on different lysates with Rat anti-GAD65 antibody (HA601389) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 60 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601389) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"