VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
Catalog# HA601393
VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
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IHC-Fr
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WB
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Species Reactivity
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr, WB
Target Molecular Weight
Predicted band size: 65 kDa
Positive Control
Mouse cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-54
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-Fr | |
|---|---|---|
| mouse |
|
|
| rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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WB
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1:2,000
Target
Function
Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.
Background References
1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan
2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.
Synonyms
Differentiation associated BNPI antibody
Differentiation associated Na dependent inorganic phosphate cotransporter antibody
Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
Differentiation-associated BNPI antibody
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
DNPI antibody
SLC17A6 antibody
Sodium dependent inorganic phosphate cotransporter antibody
Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
Solute carrier family 17 member 6 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
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☑ Relative expression (RE)
Western blot analysis of VGLUT2 on different lysates with Rat anti-VGLUT2 antibody (HA601393) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat lung tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601393) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"