Acetylcholinesterase Recombinant Antibody [PSH10-55] - Rat IgG1 (Chimeric)
Catalog# HA601400
Acetylcholinesterase Recombinant Antibody [PSH10-55] - Rat IgG1 (Chimeric)
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WB
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IHC-Fr
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Mouse
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Rat
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unconjugated
Overview
Product Name
Acetylcholinesterase Recombinant Antibody [PSH10-55] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Mouse ACHE aa 32-614.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-Fr
Target Molecular Weight
Predicted band size: 68 kDa
Positive Control
Mouse striatum tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-55
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| mouse |
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|
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| rat |
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|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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IHC-Fr
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1:500
Target
Function
Acetylcholinesterase (HGNC symbol ACHE; EC 3.1.1.7; systematic name acetylcholine acetylhydrolase), also known as AChE, AChase or acetylhydrolase, is the primary cholinesterase in the body. It is an enzyme that catalyzes the breakdown of acetylcholine and some other choline esters that function as neurotransmitters. It is found at mainly neuromuscular junctions and in chemical synapses of the cholinergic type, where its activity serves to terminate cholinergic synaptic transmission. It belongs to the carboxylesterase family of enzymes. It is the primary target of inhibition by organophosphorus compounds such as nerve agents and pesticides.
Background References
1. Rajagopalan V et al. Acetylcholinesterase biosensors for electrochemical detection of neurotoxic pesticides and acetylcholine neurotransmitter: A literature review. Environ Res. 2023 Jun
2. Rabeler C et al. Acetylcholinesterase Activity Staining in Freshwater Planarians. Curr Protoc. 2023 Feb
Subcellular Location
Synapse, Secreted, Cell membrane.
Synonyms
ACEE antibody
ACES_HUMAN antibody
Acetylcholinesterase antibody
AChE antibody
Apoptosis related acetylcholinesterase antibody
ARACHE antibody
N ACHE antibody
N-ACHE antibody
YT antibody
YT blood group antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
Application: IHC-Fr
Species: Rat
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
☑ Relative expression (RE)
Western blot analysis of Acetylcholinesterase on different lysates with Rat anti-Acetylcholinesterase antibody (HA601400) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane)
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601400) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"