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TBR1 Recombinant Antibody [JF10-00] - Rat IgG1 (Chimeric)

Usd: 315

Specification

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Catalog# HA601422

TBR1 Recombinant Antibody [JF10-00] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

TBR1 Recombinant Antibody [JF10-00] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human TBR1 aa 30-75 / 682.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P, WB

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

E14.5 rat embryo tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JF10-00

Reactivity Data

IHC-Fr IHC-P WB
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Pig
Published
Published
Published

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

A novel murine and human gene, TBR-1, encodes a putative transcription factor related to the Brachyrury (T) gene that is expressed only in postmitotic cells. T-brain-1 (TBR-1) mRNA is largely restricted to the cerebral cortex, where, during embryogenesis, it defines different regions that give rise to the palecortex, limbic cortex and neocortex. TBR-1, Pax-6 and Emx-1 are expressed in the mouse and chicken pallium. The pallio-subpallial boundary lies at the interface between the TBR-1 and Dlx-2 expression domains. Chicken genes homolgous to these mouse genes are expressed in topologically comparable patterns during development, suggesting that mouse and chicken may have similar histogenetic specification processes and field homologies. CASK/LIN-2, a membrane-associated guanylate kinase, is required for EGFR localization and signaling. In adult rat brain, CASK is concentrated at neuronal synapses and binds to the cell-surface proteins. CASK can interact with TBR-1, which is involved in forebrain development. CASK enters into the nucleus and binds to a specific DNA sequence (the T-element) in a complex with TBR-1. Thus, CASK acts as a coactivator of TBR-1 to induce transcription of T-element containing genes, including reelin.

Background References

1. Crespo I et al. Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex. Mol Neurobiol. 2022 Sep

2. Sollis E et al. Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions. Hum Mol Genet. 2023 Apr

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q64336 Mouse

Entrez Gene:680427 Rat

Synonyms

T box brain 1 antibody

T box brain protein 1 antibody

T brain 1 protein antibody

T-box brain protein 1 antibody

T-brain-1 antibody

TBR 1 antibody

TBR-1 antibody

Tbr1 antibody

TBR1_HUMAN antibody

TES 56 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex (P0)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex (P0)

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded E14.5 rat embryo tissue with Rabbit anti-TBR1 antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded E14.5 rat embryo tissue with Rabbit anti-TBR1 antibody (HA601422) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TBR1 antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TBR1 antibody (HA601422) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TBR1 on different lysates with Rat anti-TBR1 antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse liver tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 74 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601422" style="font-weight: bold;text-decoration: underline;">HA601422</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TBR1 on different lysates with Rat anti-TBR1 antibody (HA601422) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate (negative)
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601422) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"