Caspase-4 Mouse Monoclonal Antibody [A9G2]
Catalog# HA601431
Caspase-4 Mouse Monoclonal Antibody [A9G2]
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WB
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Human
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Cynomolgus monkey
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unconjugated
Overview
Product Name
Caspase-4 Mouse Monoclonal Antibody [A9G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Caspase 4 aa 81-270.
Species Reactivity
Human (Predicted: Cynomolgus monkey)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
THP-1 cell lysate, U-937 cell lysate.
Conjugation
unconjugated
Clone Number
A9G2
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue (LEVD-), and belongs to a family of cysteine proteases called caspases. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system. The anti-inflammatory drug indoprofen is an inhibitor of the activity of the caspase-4 enzyme.
Background References
1. Shi X et al. Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome. Nature. 2023 Dec
2. Wu Y et al. Caspase-4/11-Mediated Pulmonary Artery Endothelial Cell Pyroptosis Contributes to Pulmonary Arterial Hypertension. Hypertension. 2022 Mar
Subcellular Location
Cytoplasm, cytosol, Endoplasmic reticulum membrane, Mitochondrion, Inflammasome, Secreted .
UNIPROT
Synonyms
Apoptotic cysteine protease Mih1/TX antibody
CASP-4 antibody
CASP4 antibody
CASP4_HUMAN antibody
Caspase 4 apoptosis related cysteine peptidase antibody
Caspase-4 subunit 2 antibody
Caspase4 antibody
ICE(rel)-II antibody
ICE(rel)II antibody
ICEREL II antibody
ExpandImages
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Western blot analysis of Caspase-4 on different lysates with Mouse anti-Caspase-4 antibody (HA601431) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: U-937 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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