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VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)

Usd: 350

Specification

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Catalog# HA601449

VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)

Application

  • IHC-Fr

  • WB

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Mouse VGLUT1 aa 1-560.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB, IF-Tissue

Target Molecular Weight

Predicted band size: 62 kDa

Positive Control

Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-53

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

  • IF-Tissue

  • 1:500

Target

Function

Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.

Background References

1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan

2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.

UNIPROT

SwissProt: Q3TXX4 Mouse

SwissProt: Q62634 Rat

Synonyms

BNPI antibody

Brain specific Na (+) dependent inorganic phosphate cotransporter antibody

Brain specific Na dependent inorganic phosphate cotransporter antibody

Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody

Slc17a7 antibody

Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody

Solute carrier family 17 member 7 antibody

Vesicular glutamate transporter 1 antibody

VGLU1_HUMAN antibody

VGLUT 1 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (VGLUT1, <a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, Guinea pig, red); 1/500 (VGLUT2, <a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>, Mouse, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (VGLUT1, <a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, Guinea pig, red); 1/500 (VGLUT2, <a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>, Mouse, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green)

    Antigen retrieval: Not required

  • Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)<br />Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (HA601449) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
    Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601449) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Hippocampus<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: VGLUT1, 1/500 (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, Guinea pig, Red), GAD67, 1:500 (<a href="/products/HA601370" style="font-weight: bold;text-decoration: underline;">HA601370</a>, Mouse, Green), overnight at 4℃.<br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), Goat anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Hippocampus
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Rat<br />Tissue: Hippocampus<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: VGLUT1, 1/500 (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, Guinea pig, Red), GAD67, 1:500 (<a href="/products/HA601370" style="font-weight: bold;text-decoration: underline;">HA601370</a>, Mouse, Green), overnight at 4℃.<br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), Goat anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Hippocampus
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"